Coding
adhB

Part:BBa_K173017:Design

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence encoded by this gene has been found in NCBI Entrez Protein retrieval system. The organism was Zymomonas mobilis ZM4. The gene sequence has been optimized for the codon usage of Escherichia coli and a second stop codon (TAA) has been added to the gene.

Source

Mr Gene synthesis service.

References

[1] L O Ingram, T Conway, D P Clark, G W Sewell, and J F Preston, "Genetic engineering of ethanol production in Escherichia coli". Appl Environ Microbiol. 1987 October; 53(10): 2420–2425.

[2] Y A Osman and L 0 Ingram, "Zymomonas mobilis mutants with an increased rate of alcohol production". Applied and Environmental Microbiology, July 1987, p. 1425-1432 53, No. 7.